Quantitative proteomics is a powerful approach used for both the discovery and targeted proteomic analyses to understand global proteomic dynamics in a cell, tissue or organism. Most quantitative proteomic analyses entail the isotopic labelling of proteins or peptides in the experimental groups, which can then be differentiated based on mass spectrometry. Relative quantitation methods (SILAC, ICAT, ICPL and isobaric tags) are used to compare protein or peptide abundance between samples, while spiking the unlabeled samples with known concentrations of isotopically-labeled synthetic peptides can yield absolute quantification of target peptides via Selected reaction monitoring(SRM).
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